small interference rna Search Results


small interference rna targeting circ_cdr1as  (Keygen Biotech)
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ets-1 cdna  (Shanghai GenePharma)
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small interference rna targeting circ_0061395  (Shanghai GenePharma)
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p53 depletion small interference rna (sirna) sequence target p53  (Shanghai GenePharma)
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custom pool of small interference rna (sirnas)  (Shanghai GenePharma)
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Custom Pool Of Small Interference Rna (Sirnas), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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shrnas targeting loxl2  (Shanghai GenePharma)
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small interference rna to nod2 (sirna-nod2)  (Biomics Biotechnologies)
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small interference rna for fbxl2  (Shanghai GenePharma)
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small interference rna targeting raf1  (Keygen Biotech)
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Keygen Biotech small interference rna targeting raf1
Circ_CDR1as and <t>Raf1</t> are highly enriched in HCC tissues. ( A and B ) QRT-PCR assay for the relative expression of circ_CDR1as and Raf1 mRNA in HCC tissues and adjacent normal tissues (n=105). ( C ) Pearson correlation analysis for the correlation between the levels of circ_CDR1as and Raf1 in HCC tissues, r=0.4849, P < 0.0001. ( D ) Western blot analysis for the level of Raf1 protein in HCC tissues and adjacent normal tissues. * P < 0.05.
Small Interference Rna Targeting Raf1, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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small interference rna against circ_0005962  (Sangon Biotech)
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Sangon Biotech small interference rna against circ_0005962
Circ_CDR1as and <t>Raf1</t> are highly enriched in HCC tissues. ( A and B ) QRT-PCR assay for the relative expression of circ_CDR1as and Raf1 mRNA in HCC tissues and adjacent normal tissues (n=105). ( C ) Pearson correlation analysis for the correlation between the levels of circ_CDR1as and Raf1 in HCC tissues, r=0.4849, P < 0.0001. ( D ) Western blot analysis for the level of Raf1 protein in HCC tissues and adjacent normal tissues. * P < 0.05.
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bach2 sirna fragment (5’-gcacactacagcggtctta-3)  (Genechem)
90
Genechem bach2 sirna fragment (5’-gcacactacagcggtctta-3)
Circ_CDR1as and <t>Raf1</t> are highly enriched in HCC tissues. ( A and B ) QRT-PCR assay for the relative expression of circ_CDR1as and Raf1 mRNA in HCC tissues and adjacent normal tissues (n=105). ( C ) Pearson correlation analysis for the correlation between the levels of circ_CDR1as and Raf1 in HCC tissues, r=0.4849, P < 0.0001. ( D ) Western blot analysis for the level of Raf1 protein in HCC tissues and adjacent normal tissues. * P < 0.05.
Bach2 Sirna Fragment (5’ Gcacactacagcggtctta 3), supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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small interference rna targeting rcc2  (Ribobio co)
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Ribobio co small interference rna targeting rcc2
Circ_CDR1as and <t>Raf1</t> are highly enriched in HCC tissues. ( A and B ) QRT-PCR assay for the relative expression of circ_CDR1as and Raf1 mRNA in HCC tissues and adjacent normal tissues (n=105). ( C ) Pearson correlation analysis for the correlation between the levels of circ_CDR1as and Raf1 in HCC tissues, r=0.4849, P < 0.0001. ( D ) Western blot analysis for the level of Raf1 protein in HCC tissues and adjacent normal tissues. * P < 0.05.
Small Interference Rna Targeting Rcc2, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Circ_CDR1as and Raf1 are highly enriched in HCC tissues. ( A and B ) QRT-PCR assay for the relative expression of circ_CDR1as and Raf1 mRNA in HCC tissues and adjacent normal tissues (n=105). ( C ) Pearson correlation analysis for the correlation between the levels of circ_CDR1as and Raf1 in HCC tissues, r=0.4849, P < 0.0001. ( D ) Western blot analysis for the level of Raf1 protein in HCC tissues and adjacent normal tissues. * P < 0.05.

Journal: Cancer Management and Research

Article Title: CircRNA CDR1as/miR-1287/Raf1 Axis Modulates Hepatocellular Carcinoma Progression Through MEK/ERK Pathway

doi: 10.2147/CMAR.S252679

Figure Lengend Snippet: Circ_CDR1as and Raf1 are highly enriched in HCC tissues. ( A and B ) QRT-PCR assay for the relative expression of circ_CDR1as and Raf1 mRNA in HCC tissues and adjacent normal tissues (n=105). ( C ) Pearson correlation analysis for the correlation between the levels of circ_CDR1as and Raf1 in HCC tissues, r=0.4849, P < 0.0001. ( D ) Western blot analysis for the level of Raf1 protein in HCC tissues and adjacent normal tissues. * P < 0.05.

Article Snippet: To silence circ_CDR1as or Raf1, small interference RNA (siRNA) targeting circ_CDR1as (si-circ_CDR1as) or Raf1 (si-Raf1) and the negative control (si-NC) are synthesized by KeyGEN Biotech (Nanjing, China).

Techniques: Quantitative RT-PCR, Expressing, Western Blot

Raf1 depletion inhibits HCC cell proliferation and metastasis. ( A and B ) QRT-PCR and Western blot assays for the relative expression of Raf1 in THLE-2, Hep3B and Huh7 cells. ( C – J ) Hep3B and Huh7 cells are introduced with si-NC or si-Raf1. ( C and D ) QRT-PCR and Western blot assays for the relative expression of Raf1 in treated cells. ( E ) Colony formation assay for the number of the colonies formed by treated cells. ( F and G ) MTT assay for the OD value of treated cells at 490 nm. ( H and I ) Transwell assay for the migration and invasion abilities of treated cells. ( J ) Western blot analysis for the protein levels of snail and E-cadherin in treated cells. * P < 0.05.

Journal: Cancer Management and Research

Article Title: CircRNA CDR1as/miR-1287/Raf1 Axis Modulates Hepatocellular Carcinoma Progression Through MEK/ERK Pathway

doi: 10.2147/CMAR.S252679

Figure Lengend Snippet: Raf1 depletion inhibits HCC cell proliferation and metastasis. ( A and B ) QRT-PCR and Western blot assays for the relative expression of Raf1 in THLE-2, Hep3B and Huh7 cells. ( C – J ) Hep3B and Huh7 cells are introduced with si-NC or si-Raf1. ( C and D ) QRT-PCR and Western blot assays for the relative expression of Raf1 in treated cells. ( E ) Colony formation assay for the number of the colonies formed by treated cells. ( F and G ) MTT assay for the OD value of treated cells at 490 nm. ( H and I ) Transwell assay for the migration and invasion abilities of treated cells. ( J ) Western blot analysis for the protein levels of snail and E-cadherin in treated cells. * P < 0.05.

Article Snippet: To silence circ_CDR1as or Raf1, small interference RNA (siRNA) targeting circ_CDR1as (si-circ_CDR1as) or Raf1 (si-Raf1) and the negative control (si-NC) are synthesized by KeyGEN Biotech (Nanjing, China).

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Colony Assay, MTT Assay, Transwell Assay, Migration

Circ_CDR1as targets miR-1287, an upstream miRNA of Raf1. ( A ) The binding sites of miR-1287 on circ_CDR1as, as well as the mutant sites based on starBase. ( B and C ) Dual-luciferase reporter assay for the luciferase activities of circ_CDR1as-wt and circ_CDR1as-mut in Hep3B and Huh7 cells. ( D ) QRT-PCR assay for the relative expression of miR-1287 in Hep3B and Huh7 cells introduced with si-NC, si-circ_CDR1as, Vector or circ_CDR1as. ( E ) QRT-PCR assay the relative expression of miR-1287 in THLE-2, Hep3B and Huh7 cells. ( F ) QRT-PCR assay for the relative expression of miR-1287 in HCC tissues and adjacent normal tissues (n=105). ( G ) Pearson correlation analysis for the correlation between the levels of miR-1287 and circ_CDR1as in HCC tissues, r=−0.4748, P < 0.0001. ( H ) The binding sites of miR-1287 on the 3ʹUTR of Raf1, as well as the mutant sites based on starBase. ( I and J ) Dual-luciferase reporter assay for the luciferase activities of Raf1-wt and Raf1-mut in Hep3B and Huh7 cells. ( K – M ) Hep3B and Huh7 cells are transfected with miR-NC, miR-1287, anti-miR-NC or anti-miR-1287. ( K and L ) QRT-PCR assay the relative expression levels of miR-1287 and Raf1 in transfected cells. ( M ) Western blot analysis for the protein level of Raf1 in transfected cells. ( N ) Pearson correlation analysis for the correlation between the levels of miR-1287 and Raf1 in HCC tissues, r=−0.5270, P < 0.0001. * P < 0.05.

Journal: Cancer Management and Research

Article Title: CircRNA CDR1as/miR-1287/Raf1 Axis Modulates Hepatocellular Carcinoma Progression Through MEK/ERK Pathway

doi: 10.2147/CMAR.S252679

Figure Lengend Snippet: Circ_CDR1as targets miR-1287, an upstream miRNA of Raf1. ( A ) The binding sites of miR-1287 on circ_CDR1as, as well as the mutant sites based on starBase. ( B and C ) Dual-luciferase reporter assay for the luciferase activities of circ_CDR1as-wt and circ_CDR1as-mut in Hep3B and Huh7 cells. ( D ) QRT-PCR assay for the relative expression of miR-1287 in Hep3B and Huh7 cells introduced with si-NC, si-circ_CDR1as, Vector or circ_CDR1as. ( E ) QRT-PCR assay the relative expression of miR-1287 in THLE-2, Hep3B and Huh7 cells. ( F ) QRT-PCR assay for the relative expression of miR-1287 in HCC tissues and adjacent normal tissues (n=105). ( G ) Pearson correlation analysis for the correlation between the levels of miR-1287 and circ_CDR1as in HCC tissues, r=−0.4748, P < 0.0001. ( H ) The binding sites of miR-1287 on the 3ʹUTR of Raf1, as well as the mutant sites based on starBase. ( I and J ) Dual-luciferase reporter assay for the luciferase activities of Raf1-wt and Raf1-mut in Hep3B and Huh7 cells. ( K – M ) Hep3B and Huh7 cells are transfected with miR-NC, miR-1287, anti-miR-NC or anti-miR-1287. ( K and L ) QRT-PCR assay the relative expression levels of miR-1287 and Raf1 in transfected cells. ( M ) Western blot analysis for the protein level of Raf1 in transfected cells. ( N ) Pearson correlation analysis for the correlation between the levels of miR-1287 and Raf1 in HCC tissues, r=−0.5270, P < 0.0001. * P < 0.05.

Article Snippet: To silence circ_CDR1as or Raf1, small interference RNA (siRNA) targeting circ_CDR1as (si-circ_CDR1as) or Raf1 (si-Raf1) and the negative control (si-NC) are synthesized by KeyGEN Biotech (Nanjing, China).

Techniques: Binding Assay, Mutagenesis, Luciferase, Reporter Assay, Quantitative RT-PCR, Expressing, Plasmid Preparation, Transfection, Western Blot

Circ_CDR1as depletion represses HCC cell proliferation and metastasis by sponging miR-1287 to downregulate Raf1. Hep3B and Huh7 cells are introduced with si-NC, si-circ_CDR1as, si-circ_CDR1as+anti-miR-NC, si-circ_CDR1as+anti-miR-1287, si-circ_CDR1as+pcDNA or si-circ_CDR1as+Raf1. ( A and B ) QRT-PCR and Western blot assays for the relative expression of Raf1 in treated cells. ( C ) Colony formation assay for the number of the colonies formed by treated cells. ( D and E ) MTT assay for the OD value of treated cells at 490 nm. ( F and G ) Transwell assay for the migration and invasion abilities of treated cells. ( H ) Western blot analysis for the protein levels of snail and E-cadherin in treated cells. * P < 0.05.

Journal: Cancer Management and Research

Article Title: CircRNA CDR1as/miR-1287/Raf1 Axis Modulates Hepatocellular Carcinoma Progression Through MEK/ERK Pathway

doi: 10.2147/CMAR.S252679

Figure Lengend Snippet: Circ_CDR1as depletion represses HCC cell proliferation and metastasis by sponging miR-1287 to downregulate Raf1. Hep3B and Huh7 cells are introduced with si-NC, si-circ_CDR1as, si-circ_CDR1as+anti-miR-NC, si-circ_CDR1as+anti-miR-1287, si-circ_CDR1as+pcDNA or si-circ_CDR1as+Raf1. ( A and B ) QRT-PCR and Western blot assays for the relative expression of Raf1 in treated cells. ( C ) Colony formation assay for the number of the colonies formed by treated cells. ( D and E ) MTT assay for the OD value of treated cells at 490 nm. ( F and G ) Transwell assay for the migration and invasion abilities of treated cells. ( H ) Western blot analysis for the protein levels of snail and E-cadherin in treated cells. * P < 0.05.

Article Snippet: To silence circ_CDR1as or Raf1, small interference RNA (siRNA) targeting circ_CDR1as (si-circ_CDR1as) or Raf1 (si-Raf1) and the negative control (si-NC) are synthesized by KeyGEN Biotech (Nanjing, China).

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Colony Assay, MTT Assay, Transwell Assay, Migration

Circ_CDR1as depletion inactivates MEK/ERK pathway by regulating miR-1287/Raf1 axis. Hep3B and Huh7 cells are introduced with si-NC, si-circ_CDR1as, si-circ_CDR1as+anti-miR-NC, si-circ_CDR1as+anti-miR-1287, si-circ_CDR1as+pcDNA or si-circ_CDR1as+Raf1. ( A and B ) Western blot analysis for the protein levels of MEK1, p-MEK1, ERKs and p-ERKs in treated cells. * P < 0.05.

Journal: Cancer Management and Research

Article Title: CircRNA CDR1as/miR-1287/Raf1 Axis Modulates Hepatocellular Carcinoma Progression Through MEK/ERK Pathway

doi: 10.2147/CMAR.S252679

Figure Lengend Snippet: Circ_CDR1as depletion inactivates MEK/ERK pathway by regulating miR-1287/Raf1 axis. Hep3B and Huh7 cells are introduced with si-NC, si-circ_CDR1as, si-circ_CDR1as+anti-miR-NC, si-circ_CDR1as+anti-miR-1287, si-circ_CDR1as+pcDNA or si-circ_CDR1as+Raf1. ( A and B ) Western blot analysis for the protein levels of MEK1, p-MEK1, ERKs and p-ERKs in treated cells. * P < 0.05.

Article Snippet: To silence circ_CDR1as or Raf1, small interference RNA (siRNA) targeting circ_CDR1as (si-circ_CDR1as) or Raf1 (si-Raf1) and the negative control (si-NC) are synthesized by KeyGEN Biotech (Nanjing, China).

Techniques: Western Blot

Circ_CDR1as depletion suppresses HCC tumor growth in vivo. Hep3B cells stably transfected with sh-circ_CDR1as or sh-NC are subcutaneously injected into BALB/c nude mice. ( A ) The volume of formed tumors. ( B ) The weight of formed tumors. ( C – E ) QRT-PCR assay for the relative expression of circ_CDR1as, miR-1287 and Raf1 in formed tumors. ( F ) Western blot analysis for the protein level of Raf1 in formed tumors. * P < 0.05.

Journal: Cancer Management and Research

Article Title: CircRNA CDR1as/miR-1287/Raf1 Axis Modulates Hepatocellular Carcinoma Progression Through MEK/ERK Pathway

doi: 10.2147/CMAR.S252679

Figure Lengend Snippet: Circ_CDR1as depletion suppresses HCC tumor growth in vivo. Hep3B cells stably transfected with sh-circ_CDR1as or sh-NC are subcutaneously injected into BALB/c nude mice. ( A ) The volume of formed tumors. ( B ) The weight of formed tumors. ( C – E ) QRT-PCR assay for the relative expression of circ_CDR1as, miR-1287 and Raf1 in formed tumors. ( F ) Western blot analysis for the protein level of Raf1 in formed tumors. * P < 0.05.

Article Snippet: To silence circ_CDR1as or Raf1, small interference RNA (siRNA) targeting circ_CDR1as (si-circ_CDR1as) or Raf1 (si-Raf1) and the negative control (si-NC) are synthesized by KeyGEN Biotech (Nanjing, China).

Techniques: In Vivo, Stable Transfection, Transfection, Injection, Quantitative RT-PCR, Expressing, Western Blot